Auxiliary methods improving IVF chances

ICSI: selection of a single sperm cell

ICSI method is usually offered to couples in following cases:

  • Poor quality of sperm.
  • Presence of antisperm antibodies.
  • Use of cryopreserved sperm of low quality.
  • Age of a female patient over 38 years.
  • Severe endometriosis.
  • Infertility of unknown genesis.

 

Selection of a single viable sperm cell from the partner’s sample is performed and this sperm cell is introduced with a special needle directly into the egg. In other words, unlike IVF, conception occurs not with a random sperm cell, but in laboratory conditions under full control by a fertility specialist.

The number of fertilized eggs after ICSI reaches 70%. About 80% of them begin dividing normally.

 

IMSI: chances improve

With the use of this method, before introduction to an egg, a sperm cell is subjected to screening in a laboratory under a microscope with high magnifying power. Just compare: ICSI employs a microscope with 400-fold magnification, whereas IMSI – with 6000-fold magnification. In this case, morphology of a sperm cell and its appropriate structure are considered in the first place.

 

PICSI: selection of a mature sperm cell

The method hardly differs from ICSI and can be used in combination with IMSI.

The method hardly differs from ICSI and can be used in combination with IMSI.

The sperm cell, which is selected for fertilization by a fertility specialist-embryologist, undergoes additional “test for maturity”. Before fertilization, sperm cells are immersed in a tube containing hyaluronic acid. It is assumed that those sperm cells that interact with the acid have less DNA abnormalities and are optimal in terms of morphology (i.e. their structure) and have reached their mature phase. It is not possible to visually determine maturity of a sperm cell even with multiple magnification.



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