Treatment is legal. Stem Cell Therapy is permitted by Ukrainian law.
We employ the services of a healthcare lawyer for appropriate legal backing of the process and execution of contracts.
Treatment is carried out in accordance with guidelines and recommendations of the American Society for Cell Biology.
We consult with the experts from the Medical University of Graz (Austria) and other European institutions.
We act as partners of the Eastern Europe’s only biobank – the member of the ESBB (European, Middle East & African Society for Biopreservation and Biobanking).
The laboratory has the ISO 9001:2015 Certificate.
All analyses are performed in accordance with the GLP principles.
We use methods approved by the U.S. Food and Drug Administration (FDA).
Before starting the stem cell therapy, we conduct cancer screening using blood tests. Click here to learn more
There is a possibility of video monitoring of the process of stem cell culture in our laboratory.
ABOUT STEM CELLS
A human body consists of more than 200 types of cells, each of these cell types originate from a zygote, which is the only cell formed when the egg cell is fertilized by sperm.
During several days, this separate cell is divided over and over again, until it forms a blastocyst, a sphere consisting of 150-200 cells. The blastocyst gives growth to each separate type of cell that are necessary for the human body to survive, including the umbilical cord and placenta feeding the developing fetus.
Stem cells are the basis for the development of plants, animals, and humans. Stem cells are the basis of each organ and tissue of our body.
Humans have many different types of stem cells that originate from different sites of the body and form at different times throughout our lives.
They include embryonic stem cells that exist only at the earliest stages of development and various types of tissue-specific (or adult) stem cells that occur during fetal development and remain in our bodies throughout life.
Stem cells are defined by the following two characteristics:
- They can make copies of themselves or, in other words, self-renew
- They can differentiate or, in other words, develop into more specialized cells
Embryonic stem cells are pluripotent, i.e. they can generate all types of cells of the body, but cannot create support structures, such as the placenta and umbilical cord.
Other cells are multipotent, i.e. they can generate several different types of cells, usually, in a particular tissue or organ.
As the body develops and gets old, the number and type of stem cell change. Totipotent cells are no longer present after division to cells that generate the placenta and umbilical cord.
Pluripotent cells give growth to specialized cells that constitute body organs and tissues.
Stem cells that stay in your body throughout your life are specific to tissues; and there is evidence that these cells are changing along with your age: your skin stem cells at the age of 20 would not be the same as your skin stem cells at the age of 80.
Briefly summarizing, we can say that stem cells are cells, which, among other things, represent the system of internal recovery of lost cells.
TYPES OF STEM CELLS
There are many different types of stem cells that originate from different sites of the body and form at different stages of our life. Stem cells fall into several groups: embryonic, fetal, tissue-specific postnatal (also called somatic or stem cells of an adult body), and induced pluripotent stem cells.
EMBRYONIC STEM CELLS
Embryonic stem cells (ESCs) were first isolated from the human embryo in 1998. By nature, these cells are pluripotent – in other words, they are able to form ANY tissue of the body.
ESCs occur on day 2-11 of the fertilized egg development, later on, they form several main cell lines. So, from the moment of fusion between the egg cell and the sperm cell and to the moment of the 8th division (up to formation of 256 cells) – each cell represents an embryonic stem cell, having no specialization, and can turn into any tissue of the body.
The unique character of ESCs lies in the fact that they do not produce histocompatibility antigens, i.e. when these cells are administered, they do not cause a rejection reaction; there is no incompatibility.
The disadvantage is that there are no embryonic stem cells in the body of an adult person, and, most regrettably, obtainment of embryonic stem cells requires destruction of an embryo, after which its further development is not possible (there is no way to separate only one cell; when its integrity is violated, the embryo dies inevitably).
At present, embryonic stem cells are obtained in laboratory settings (in vitro) from the inner cell mass of a blastocyst. For this purpose, the egg cell is artificially fertilized with the sperm cell, and the embryo is grown to the necessary size. Thus, embryonic stem cells are the product of IVF (in vitro fertilization).
Under moral and ethical principles, as well as by law, obtainment and use of ESCs is forbidden in many countries.
FETAL STEM CELLS
Stem cells derived from organs of embryos are also called fetal stem cells. This name comes from the word “fetus”, having Latin origin and meaning “unborn offspring, embryo”. Fetal stem cells are derived from the fetal material after an abortion or death of the fetus.
Stem cells are obtained from organs of aborted embryos not older than 22 weeks of gestation.
Stem cells are separated depending on the type of the organ, from which they were derived. For example, there are hepatic, brain, nervous cells, etc. Each pluripotent stem cell can evolve only into several types of tissues or organs. This is due to the fact that as soon as the number of cells in the embryo reaches 256 cells, primary organs and tissues begin to be distinguished in the human embryo. These primary structures will subsequently give rise to each and all organs and tissues in the human body. That way, the embryo develops mesenchymal, nervous, blood, and connective tissue pluripotent stem cells.
After 22 weeks of gestation, all stem cells have already become specialized and specific. For instance, a hepatic stem cell can only turn into hepatic cells. In other words, cells are “assigned” to the certain type of tissue, and this means they are present only in this organ, and will never be able to move over to another type of tissue.
TISSUE-SPECIFIC STEM CELLS
A human being is born with already specialized stem cells present in every organ and tissue, which constitutes a kind of cell stock.
During a lifetime, new cells of every organ and tissue are formed from this stock in replacement of cells lost as a result of common daily living (cells of the skin, blood and intestinal surface).
Throughout life, the number of these stem cells gradually decreases; but even by the time of human physiological death related to age, they are still present in all organs and tissues.
It was previously thought that only specialized stem cells could be derived from any organ of an adult person. However, the bone marrow even of an adult person has two types of unspecialized stem cells, which are blood-forming (hematopoietic) and mesenchymal cells; these cells are now obtained and isolated in laboratory settings.
For example, hematopoietic stem cells are not able to generate brain cells or hepatic cells, while stem cells in other organs are not able to produce blood cells.
Mesenchymal stem cells are isolated from the stroma, which is a connective tissue surrounding organs. Thus, mesenchymal stem cells can also be called stromal cells. They can differentiate into bone tissue cells, cartilage cells and adipose cells. At present, main sources of mesenchymal stem cells include the bone marrow, adipose tissue and blood.
Despite the fact that stem cells of an adult body have lower potency compared to embryonic and fetal stem cells, in other words, can generate a smaller amount of different types of cells, they have an undeniable advantage – the ethical aspect of their obtainment and use does not cause serious controversy and disputes in the society.
In addition, the possibility to use autologous (individual’s own) material ensures efficacy and safety of treatment, since this does not cause allergy, incompatibility or rejection reaction.
INDUCED PLURIPOTENT STEM CELLS
In 2012, the British scientist, John B. Gurdon, and the Japanese scientist, Shinya Yamanaka, were awarded the Noble Prize in Medicine for “for the discovery that mature cells can be reprogrammed to become pluripotent”.
This gave further impetus to research development in the field of stem cell therapy.
Induced pluripotent stem cells (iPSCs) are the cells that in laboratory settings with the use of modern technologies were reprogramed by transforming tissue-specific cells (for example, skin cells) into cells that act like embryonic stem cells.
This means that iPSCs, just as embryonic stem cells, can evolve into all types of body cells.
Currently, there are plenty of methods for obtaining iPSCs, and laboratories capable of producing them possess a serious scientific potential of the global scale.
One of the major advantages of induced pluripotent stem cells is that their obtainment does not require destruction of an embryo (as is the case with embryonic stem cells) or the use of abortive (virtually dead) material.
SOURCES OF STEM CELLS
- Peripheral blood (from a vein)
- Bone marrow
- Adipose tissue of an adult person
- Umbilical cord blood of a newborn
- Material derived from women at 2-12 weeks of gestation
- Fetuses at 18-22 weeks of gestation that died due to premature delivery, immature birth or other reasons
OBTAINMENT OF STEM CELLS
- Not so long ago, the main source of stem cells was considered the bone marrow. This source is still used today.
Stem cells are located in the bone marrow of such bones as femurs, ribs, the sternum and the iliac bone.
The bone marrow (from 20 to 200 mL) is obtained by puncture of the iliac bone in adults or of the sternum in children.
- Peripheral blood is withdrawn from a vein to a sterile tube, as during a routine blood test. The procedure is absolutely painless, and the required amount of blood is 10-20 mL.
- Adipose tissue is collected under local anesthesia from the adipose layer of about 5 mL of fat.
- Umbilical cord blood is simply collected to a sterile tube directly at the maternity hospital by placing the tube under the cut cord of a newborn.
The concentration of stem cells in umbilical cord blood is ten times higher than in the bone marrow.
- Not so long ago, the main source of stem cells was considered the bone marrow. This source is still used today.
- Stem cells of umbilical cord blood possess high activity and are capable of rapid growth and specialization.
- The use of autologous umbilical cord blood guarantees a 100% match. The chance of matching with siblings is 25%, that is why it is possible to use child’s umbilical cord blood to treat other close blood relatives.
However, in umbilical cord blood, hematopoietic (blood-forming) stem cells are prevailing. Mesenchymal stem cells are contained in umbilical cord blood in much smaller amounts.
Afterwards, the material obtained by any of the above methods is transferred to the laboratory, where stem cells are isolated from it.
Cells, obtained and isolated by special techniques, are collected into a sterile tube, a growth medium is added to it, and they are separated with a centrifuge several times to remove all accidentally caught cells that do not belong to stem cells.
The prepared pool of stem cells is placed in the growth medium for further growing (culturing).
When a sufficient amount of stem cells is obtained, they are frozen in liquid nitrogen to be stored for an unlimited period or they are administered to the patient according to a special technique. Administration can be done both intravenously and locally (e.g., to a joint), depending on the treatment regimen prescribed by a physician.
GROWING OF STEM CELLS
This procedure is usually called “stem cell culturing”. Culturing of stem cells is carried out in special solutions containing nutrients (growth media). It is of great importance, which growth media are used for culture. The better the quality of the medium (and, consequently, the more expensive it is), the greater amount of stem cells is obtained.
Usually, stem cells are cultured until their quantity is sufficient to perform the therapeutic injection or to freeze them (cryopreserve) in liquid nitrogen.
Cryopreservation allows to keep the obtained stem cells for repeated procedures (performed in a year or two). In this case, the cost of subsequent administrations will be lower due to lower expenses on growth media for cell culture.
USE OF STEM CELLS
At present, there are three main fields of the use of stem cells:
- Treatment of different diseases
- Rejuvenation; improvement of the general state of health and body tonus
- Experimental clinical studies
The field of experimental research accounts for at least 80-90% of the total amount of stem cells used. This field of use of stem cells is making a steady progress: biologists regularly report on new achievements and discoveries, which means new opportunities.
There are expectations that the problem of growing donor organs for people requiring transplantation will be solved soon. In everyday practice, the use of vascular and cardiac valves grown from stem cells is gradually becoming a reality.
Stem cells are increasingly used to treat diseases. Results of observations form the basis for developing the safest and most effective clinical protocols approved by the world community, which outline the recommended regimen for the administration of stem cells in each disease, as well as sites and routes of administration, optimal therapy duration and the anticipated effect.
For the rejuvenating purposes, stem cells are administered locally (for example, beauty injections for the face, arms, and decollete area), as well as intravenously.
Such use of stem cells allows significant reduction of visible signs of age-related changes and removal of some defects. To keep the long-term effect, it is recommended to administer stem cells repeatedly at individually selected intervals. This procedure, if done appropriately, is safe.
ROUTES OF ADMINISTRATION OF STEM CELLS
Stem cells are administered by the following main routes:
- Intravenous administration of stem cells in sterile normal saline solution according to an individualized regimen
- Subcutaneous or intracutaneous administration of stem cells – “beauty shots”
- Administration of stem cells to the vessels of the affected organ using special equipment (an angiography system)
- Administration of stem cells directly to the affected organ during a surgery
- Growing parts of organs for transplantation – for example, transplantation of the amniotic membrane or islets of Langerhans
IT IS IMPORTANT TO KNOW THIS BEFORE STARTING THE STEM CELL THERAPY
In each specific case, the method and route of administration are chosen by the physician based on the person’s general state of health and the intended effect. However, there are also regulatory guidelines ensuring treatment safety and achievement of the result.
Stem Cell Therapy leads to improvement in the person’s general state of health, allows partial restoration of the functions lost, improved quality of life, reduction in the rate of disease progression and development of complications, and in some cases, it enables complete recovery from pathologies considered very difficult to treat by means “traditional” medicine.
However, it should be kept in mind that stem cell therapy is not a panacea. Remembering this is especially important for patients with type 2 diabetes mellitus. In this case, the effect of stem cell administration directly depends on adherence to recommendations of the attending physician regarding diet correction and physical activity.
At the present stage, stem cells can be used as a supplement to traditional therapy. Thus, when considering the decision to use stem cells, you should remember that the rest of therapy for the severe chronic disease must not be canceled.
The use of stem cells will significantly improve the well-being and enhance efficacy of traditional therapy, which means, patient’s quality of life will improve as well.
MAIN AREAS OF STEM CELL THERAPY USE
The list of diseases for which stem cell therapy is effective is very long. This is explained by properties of stem cells described above, such as their ability to transform into cells of different body tissues.
- Post-traumatic changes
- Burns and scars from burns
- Consequences of compound fractures
- Cartilage destruction and fibrosis
- Disorders of the central and peripheral nervous systems
- Consequences of brain and spinal cord injuries and strokes
- Alzheimer’s disease
- Multiple sclerosis
- Parkinson’s disease
- Motor neuron disease and amyotrophic lateral sclerosis
- Autoimmune diseases
- Type 1 diabetes mellitus
- Rheumatoid arthritis
- Autoimmune thyroiditis
- Respiratory system disorders
- Chronic obstructive pulmonary disease (COPD)
- Bronchial asthma
- Chronic fatigue syndrome
- Recovery from severe diseases and surgeries
- Cardiovascular diseases
- Myocardial infarction
- Ischemic heart disease
- Immune deficiencies
- Vascular disorders
- Limb ischemia
- Reynaud’s syndrome
- Trophic ulcers
- Diabetic foot
- Hepatic disorders
- Consequences of hepatitises
- Hepatosis and hepatic steatosis
- Reproductive system disorders
- Erectile dysfunctions
- Peripheral neuropathies: diabetic, toxic, compression, chronic inflammatory (dysimmune) and demyelinating polyneuropathy
- Post-traumatic changes
- Anti Age
- Rejuvenation, improvement of the general state of health and body tonus
- Cosmetology: “beauty injections” with stem cells for the face, arms and decollete area
- Improving visual appearance
- Recovery after chemotherapy
- Trichology (alopecia)
- Ophthalmology; macular degeneration
- Maxillofacial surgery and dentistry
- Implantation surgeries
Studies in the field of medical use of stem cells are conducted on a continuous basis all over the world; for this reason, the range of diseases treated using stem cell therapy is constantly growing.
TREATMENT OF DISORDERS OF THE CENTRAL AND PERIPHERAL NERVOUS SYSTEMS
Regenerative stem cell therapy has proven effective in treating such complex progressive disorders of the nervous system as neurodegenerative processes, atrophy, cerebral atherosclerosis, consequences of strokes, multiple sclerosis, Alzheimer’s disease, Parkinson’s disease, consequences of injuries in the course of rehabilitation, myodystrophies, peripheral nerve demyelination, and many more.
In these pathologies, the principle of action of stem cells is similar: administration of tropic (specific – of a particular type) stem cells promotes intergrowth of nerve fibers. Neuronal conductivity is restored, which means that the lost motor and sensory functions are gradually regained; cicatrical regions in the brain are reduced in size, microcirculation is improved, and the capability of nerve transmission is recovered.
If the disease is not advanced, a marked progress in recovery is possible. In advanced cases, there is no talk about full recovery, although mental performance, memory, the capability of self-control and self-care are improved, and hence, the quality of life increases substantially.
USE OF STEM CELLS TO TREAT JOINTS
Administration of stem cells to articular structures facilitates regeneration of the damaged areas of tendons, ligaments, meniscuses, and cartilages. Also this leads to reduction in activity of the inflammatory process.
Stem cell therapy is able to postpone a surgery for implantation of the artificial joint or even can help to avoid it.
Stem cell treatment for arthrosis will not allow to eliminate the disease completely, however, the number of complaints of pain significantly decreases, the range of movement in the patient’s joints increases – it becomes much easier for patients to move their joints.
Joint diseases, for which the use of stem cells is indicated
- Injuries of tendons, ligaments, and muscles in athletes – for rapid recovery in cases when the use of local hormonal agents is not possible due to requirements of the anti-doping commission
- Chronic tendinosis, tendopathy, enthesopathy, and fibrosis
- Degenerative changes and thinning of the articular cartilage and destruction of the periosteum
- Osteoarthrosis and gout
- Synovitis and long-standing bursitis
- Arthroses of different genesis
- Fractures with delayed healing and recovery from major surgeries
What are evident advantages of cellular therapy?
- Absence of allergic reactions
- Full compatibility with conservative treatment – traditional treatment can be continued
- Significant acceleration in recovery after joint surgeries
- Restoration of body’s own tissues and structures
- Resolving inflammation, pain, and edema
- No need of hospitalization (the procedure is done on an outpatient basis)
It should be remembered that in the case of grade 4 arthrosis, administration of stem cells would not reduce the extent of arthrosis to the state of a new joint.
However, these patients will be able to live without pain, to care for themselves without assistance; they will no longer need to take a lot of painkillers (that can cause ulcer). Such patients will have an increased range of joint movements (their joint mobility will improve), and better night’s sleep…
Surgery and general anesthesia are often contraindicated for elderly due to cardiac disorders and other pathologies. In such cases, combination of stem cell therapy and conservative (traditional) treatment will provide a long-lasting positive result.
Diabetes mellitus is one of most widespread chronic diseases in the world. According to individual authors, 4-6% of the world population suffers from it.
At present, medicine allows for quite good compensation of this disease – people can live a full-fledged life, can study, work, have family and children, and be socially active.
However, every single day throughout their life, such patients have to put up with substantial limitations:
- The patient has to monitor blood sugar level constantly, and frequently undergo examinations not to miss the development of complications
- He/she must take care of nutrition and have a sufficient level of physical activity
- Most importantly, he/she needs to do insul-in injections several times a day or take sugar-lowering medications
The use of stem cell therapy in type 1 and 2 diabetes mellitus can help significantly improve the quality of life of patients.
Type 1 and 2 diabetes differs by the disease mechanism:
- In type 1 diabetes mellitus, cells of the pancreas do not produce insul-in
- In type 2 diabetes mellitus, insul-in is produced, although cells of the body are not able to get glucose
In both cases, the blood sugar level is increased.
Administration of stem cells eliminates this problem by improving the state of the pancreatic tissue itself: stem cells entering the body with blood flow possess tropism – they are accumulated in the pancreatic tissue, gradually restoring its structure, and the sugar level starts to decrease in a gradual manner.
In type 2 diabetes mellitus, stem cell therapy reduces resistance of body cells – glucose starts to get inside the cells.
In addition, stem cells “rescue” target organs, where new vessels begin to intergrow, and blood flow, tissue respiration, and microcirculation are accelerated and enhanced. Thus, this significantly lowers the risk of loss of vision, renal and hepatic failure, encephalopathy, and “diabetic foot”.
The course of stem cell therapy in patients helps to improve their laboratory findings, improve renal function, improve limb sensitivity, stop the process of deterioration in vision, decrease drowsiness and tiredness, normalize sleep and increase working capacity. If the disease is not advanced, blood glucose values are decreased.
CHRONIC FATIGUE SYNDROME
The stem cell therapy is highly effective for a group of symptoms and syndromes that can be classified as “changes in the general state of health”.
In the setting of chronic fatigue syndrome, this treatment ensures better and healthier sleep, increases working capacity, libido, and sexual potency.
Patients, who have received a course of stem cell therapy, report improving the quality of life, increase in vitality, slowing down premature aging, increase in life expectancy, improving memory, attention, and focusing ability.
REJUVENATION WITH AUTOLOGOUS STEM CELLS
GUARANTEED NATURAL AND SAFE REJUVENATION
Stem cells promote renewal and restoration of lost proteins and tissues of the skin (collagen and elastin), as well as stimulate regeneration of sites damaged by time.
With age, the body’s cell stock is depleted, the skin is getting old, wrinkles occur, and elasticity is reduced.
For obtaining a bright rejuvenating effect, cosmetologists developed a new rejuvenation method – injections of stem cells. This method can be called mesotherapy with stem cells.
The principle of action lies in active propagation of the administered stem cells and formation of “youth proteins”. Following administration, the cell composition of tissues is vitalized and restored, blood supply is improved, and the person’s own connective-tissue support structure is strengthened, tissue immunity is activated, tissue respiration and oxygen supply improve, and there comes the guaranteed long-lasting rejuvenating effect.
- Tired skin syndrome and deterioration of skin’s appearance
- Cicatrices and scars
- Turgor reduction and sagginess of tissues
- Age-related changes: drooping, expression lines and photoaging
Stem cell rejuvenation cannot be performed in the presence of symptoms of ARD (acute respiratory disease), herpetic fever, exacerbation of acne, exacerbation of chronic diseases, and oncopathology.
If necessary, a specialist is consulted to determine the presence of contraindications.
At a time that is convenient for the patient, a cosmetologist administers stem cells to the chosen area; the procedure and technique of administration resemble biorevitalization and mesotherapy procedures.
In the course of the procedure, a cosmetologist uses a syringe to inject more than 100 thousand cells, all of which start to propagate actively.
This is accompanied by the following changes:
- Appearance and color of the skin improves, and the relief of the skin’s surface becomes smooth
- The tissue structure is renewed and improved
- The aging process slows down
- The facial contour is lifted
- Even the deepest wrinkles are smoothed; the depth and quantity of wrinkles is reduced
- Skin firmness, tightness, and elasticity are improved
- The photoaging effect is diminished
Stem cell mesotherapy or biorevitalization can be associated with such unpleasant consequences as hematomas, bruises, swelling, and edemas in the treatment area, as it can be with traditional mesotherapy and biorevitalization.
TREATMENT OF HEPATIC DISORDERS (CIRRHOSIS, FIBROSIS, HEPATOSIS)
Today, the most advanced method for treating hepatic disorders is stem cell therapy.
It should be noted that patients usually resort to this method either when all available conservative treatment options have been pursued without achieving a stable positive result, or when the disease is progressing and liver transplantation is indicated.
That is why usually patients are already in a serious condition and are difficult to manage.
However, under these conditions as well, the use of stem cells in hepatology shows good and stable results.
The liver itself represents a unique organ capable of self-restoration, and the administration of а concentrate of specialized stem cells can significantly improve the state of the liver tissue. The extent of fibrosis decreases, high blood pressure in the portal venous system reduces, blood supply to liver tissues improves, new capillaries intergrow, the damaged structure is restored, the extent of hepatic failure decreases, the risk of fatal bleedings from varicose veins is reduced, the risk of developing liver cancer is reduced, laboratory test findings improve, appetite increases, the quality of sleep becomes better, joint pain and edemas are eliminated, yellow sickness and skin itching alleviate, and mental activity is improved…
Stem cell therapy is able to not only improve the well-being but also prolong the lives of patients with severe hepatic disorders. It is not infrequent that this is the only way to be cured and an alternative to liver transplantation.
Stem cells are used in the form of a bioplastic material, as a remedy of the systemic action.
- ХChronic trophic ulcers
- Major surgeries, excision of structures and commissures
- Treatment of degenerative-dystrophic processes and posttraumatic complications
STEM CELLS IN DENTISTRY
Often, patients seek medical advice of a dentist due to problems of gingival bleeding and tenderness, pathological tooth loosening, the risk of adentia, the need for implantation of a large number of teeth (which in itself is a highly traumatic procedure), as well as due to pathology of the temporomandibular joint, such as arthrosis and functional impairment. The specific feature of treatment of these problems is the duration of the treatment course.
Local use of stem cells in dentistry is extremely effective owing to strengthening of the support structure of soft tissues around teeth, thickening and tightening of ligaments, increase in the amount of collagen, intergrowth of a large number of capillaries, improvement of blood supply, and improvement of mechanisms of tissue respiration and oxygen saturation.
These regenerative processes allow to obtain the desired anticipated positive effect in all patients. The result is a beautiful and healthy smile, and freedom from the fear of losing teeth.
STEM CELLS IN TRICHOLOGY
Millions of men and women around the world suffer from the problem of hair shedding or complete hair loss. This significantly deteriorates the quality of life, becoming a cause of psychosomatic pathology and serious emotional stress.
Traditional treatments often give a temporary effect.
The use of stem cells has undeniable advantages over other methods, it often affects the mechanism of hair loss, has no side effects and no rejection reaction, gives indisputable results, stops the development of the disease, restores the damaged area, returns an attractive appearance, and generally improves the quality of life.